RNA affinity chromatography

This is a protocol for RNA immobilization of two in vitro synthesized RNAs (RNA A and RNA D) of respectively 61 and 73 nt on agarose beads and purification of RNA-bound proteins, according to Caputi et al. 1999

It was done with 5'end labeled RNA to test the efficiency of RNA immobilization on the agarose and the cpm counts are displayed on the right side of the excel worksheets.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=10406810

P.S.: I implemented NP40 in the washing buffer of the last downloadable protocol, allowing for more stringency than what is illustrated by these two gels.